Immunoaffinity column method of analysis for total aflatoxins
نویسندگان
چکیده
منابع مشابه
Validation of an UHPLC-MS/MS method for simultaneous analysis of 11 mycotoxins in wheat flour Using Immunoaffinity column
This study focuses on optimization and validation of an Ultrahigh-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method for simultaneous analysis of 11mycotoxins: Aflatoxin B1, T-2 Toxin, Ochratoxin A, Deoxynivalenol and Zearalenone in wheat matrix. Sample extraction and cleanup procedure is based on a single extraction step using acetonitrile/water/acetic acid mixture...
متن کاملValidation of an UHPLC-MS/MS method for simultaneous analysis of 11 mycotoxins in wheat flour Using Immunoaffinity column
This study focuses on optimization and validation of an Ultrahigh-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method for simultaneous analysis of 11mycotoxins: Aflatoxin B1, T-2 Toxin, Ochratoxin A, Deoxynivalenol and Zearalenone in wheat matrix. Sample extraction and cleanup procedure is based on a single extraction step using acetonitrile/water/acetic acid mixture...
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Several column chromatographic methods are available for the separation of aflatoxins (l-6). Most of these procedures are elaborate and involve several steps of purification. A rapid and simple method of obtaining pure aflatoxins was reported by Steyn (5); however, with this method, we were not able to obtain pure crystalline aflatoxins B, and G1 for our experimental studies on animals. When pu...
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A collaborative study was conducted to evaluate a liquid chromatographic (LC) method with immunoaffinity column cleanup for determination of ochratoxin A. The method was tested at 3 concentration levels of ochratoxin A in barley, which represent possible future European regulatory limits. The test portion was extracted with acetonitrile-water by blending at high speed. The extract was filtered,...
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ژورنال
عنوان ژورنال: Mycotoxins
سال: 2012
ISSN: 0285-1466,1881-0128
DOI: 10.2520/myco.62.127